Determination of radioactively labeled globulin turnover by the direct whole-body counting technique.

An Assay for Intercellular Adhesive Specificity

A modification of an assay for intercellular adhesive specificity is described. The method involves the collection of radioactively labeled cells by aggregates of the same (isotypic aggregates) or different (heterotypic aggregates) types of tissue and determination of the number of cells collected by liquid scintillation counting. The use of (32)P to label the tissues permitted a much more rapi...

The Use of Radioactive Lysine in Studies of Protein Metabolism

Racemic lysine labeled with C(14) in the epsilon carbon position was fed to dogs. The distribution of C(14) in blood and tissue fractions is recorded. In normal dogs sacrificed at 24 hours, approximately one-third of the C(14) was found in the urine, one-third in expired air, and one-third in the body, mostly in protein, predomantly as lysine residues. The rate of C(14) excretion as CO(2), hour...

Combined microautoradiography-16S rRNA probe technique for determination of radioisotope uptake by specific microbial cell types in situ.

We propose a novel method for studying the function of specific microbial groups in situ. Since natural microbial communities are dynamic both in composition and in activities, we argue that the microbial "black box" should not be regarded as homogeneous. Our technique breaks down this black box with group-specific fluorescent 16S rRNA probes and simultaneously determines 3H-substrate uptake by...

The determination of iron absorption and loss by whole body counting.

Application of radioimmunoassay technique for determination of antigen concentration in different cells with a new monoclonal antibody [Persian]

Introduction: Binding a monoclonal antibody to tumor associated antigens is an effective method for cancer therapy because these agents can specifically target malignant cells, in fact monoclonal antibodies are effective agents for diagnosis, grading and treatment of different kinds of cancers. Methods: In this research, a new monoclonal antibody against colon cancer cells was prepared an...